This is a List of Characters in The 100 Acre Wood series.
- Winnie the Pooh
- Sheriff Woody
- Mrs. Brisby
- Fievel Mousekewitz
- Piglet
- Jeremy the Crow
- Buzz Lightyear
- Captain Justin
- Jessie the Cowgirl
- Tigger
- Eeyore
- Rabbit
- Chip and Dale
- Jiminy Cricket
- Cholena
- Yoda
- Nicodemus
- Genie
- Owl
- Mickey Mouse
- Goofy
- Donald Duck
- Hamm
- Rex
- Slinky Dog
- Potato Head
- Timmy
- Martin
- Teresa
- Cynthia
- Tiger the Cat
- Mr. Ages
- Tanya Mousekewitz
- Tony Toponi
- Umpy the Chimpanzie
- Monterey Jack
- Gadget Hackwrench
- Abu the Monkey
- Iago the Parrot
- Pete Junior
- The Green Ailiens
- Baloo the Bear
- King Louie
- Roo
- Kanga
- Alex
- Marty
- Melman
- Gloria
- Simba
- Pumbaa
- Timon
- Nala
- The Penguins Of Madagascar
- King Julian
- Maurice
- Mort
- Marlene
- Alpha Beta and Gamma
- Gopher
- Gregory Mouse
- Brutus
- Miss Right
- Napoleon and Lafayette