It is a cross-over between Alvin and the Chipmunks and the Transformers media franchise (specifically the 2007 live-action motion picture, adding elements of the Transformers: Generation 1 continuity).
It was also inspired in part by actual events-specifically the passing of Star Trek actress Majel Barrett-Roddenberry. References to her death were worked into the story-a radio news report on Barrett-Roddenberry's death plays and a character born during the story's events is named after the so-called "First Lady of Star Trek."
The story begins with an alternate version of the final scene of Wildcat Spirits Forever!-Johnny 5 and the "Three Stooges" robots leave the Astro MegaShip to investigate Tranquility (a town on the California/Nevada border and where the 2007 Transformers motion picture is set). This is because Johnny has detected a locator signal.
Eventually, the robots trace the source of the signal to a junkyard where they find Number 4-the robot controlled by Dr. Jacques von Hamsterviel in Ohana, Interrupted. We are then introduced to the Autobots, a group of heroic warriors from the long-dead planet Cybertron. Using Number 4 (who took the name "Jazz," after a fallen Autobot warrior), the Autobots are seeking help against their rivals-the evil Decepticons-who have stolen a prototype spaceship from the same planet where the Astro MegaShip II was built.
Johnny summons Alvin and Brittany to the junkyard, where they meet Sam and Mikela-the human protagonists of the 2007 Transformers movie-and where we find out that Chipmunk-1-Team Chipmunk's primary land transport-is an Autobot (Ultra Magnus). Team Chipmunk agrees to help.
The day after the "Chipmunk Adventure 20th Anniversary Tour's" concert in Tranquility, Team Chipmunk receives a distress call from a pair of Autobot warriors who survived an attack by the Decepticons on Earth's moon. They travel to the Moon to rescue the Autobots.
However, a battle ensues which culminates in the death of Optimus Prime. After a somber burial, life goes on for Team Chipmunk as they return to Earth to continue the Tour.
Alvin, Simon, Theodore, Brittany, Jeanette, Eleanor-Owned by Bagdasarian Productions, Inc.
Max Goof, Roxanne Martin-Goof, Lizzie McGuire, Ren and Louis Stevens, Kim Possible, Ron Stoppable, Rufus, Launch (Experiment 607), Kelsi Nielsen, Troy Bolton, Gabriella Montez, Ryan Evans-Owned by Disney Enterprises, Inc.
Jett Jackson-Owned by Echo Bridge Entertainment (as successor-in-interest to Alliance Atlantis Communications, Inc.) and Disney Enterprises, Inc.
Jason Archer, Melinda Crosby, Zeo (Jookiba Experiment Offspring Alpha), Padme Goof, Majel Goof-Owned by the Author.
Johnny 5-Owned by TriStar Pictures.
All The Price is Right references-Owned by Fremantle Media North America, Inc.
Rebecca Hawkins-Owned by Studio Gallop and Kazuki Takahashi (U.S. character name owned by 4Kids Entertainment, Inc.).
All Power Rangers references-Owned by SCG Power Rangers, LLC./Toei Company, Ltd.
Yubaba (Spirited Away)-Owned by Studio Ghibli Co., Ltd.
Thom E. Gemcity (a/k/a Tim McGee) (N.C.I.S.)-Owned by CBS Studios, Inc.
All Transformers references-Owned by Hasbro Toys, Inc.
Characters from the 2007 Transformers movie-Owned by Paramount Pictures/Dreamworks SKG.